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Research Assistant Pediatrics

Company: University of Tennessee
Location: Memphis
Posted on: December 5, 2019

Job Description:

Education - Bachelor's Degree Skills - Transfection - Aseptic Technique - Western Blot - Data Entry - Communication Skills Pay Grade: 36 Hiring Range: $15.01- $18.01 THIS IS A GRANT-FUNDED POSITION. JOB SUMMARY/ESSENTIAL JOB FUNCTIONS: The Research Assistant performs research on different novel knock-in and knock-out mouse models of human cardiomyopathy/ heart failure in the Pediatrics Department. Maintains animal colonies, performs genotyping/phenotyping, and assists in performing non-surgical and surgical procedures on animals. Assists with primary cell isolations, cultures, and the maintenance of a large research database. DUTIES AND RESPONSIBILITIES: Performs animal non-surgical and surgical procedures including handling animals, genotyping (ear punching and tail cut), tissue collection, perfusion, phenotyping, stress tests, and assists in surgical procedures. Extracts genomic DNA from mouse-tails, Polymerase Chain Reactions. Prepares media and reagents for cell culture/animal work. Maintains sterilization of cell culture materials and surgical tools/supplies. Assists in basic laboratory techniques such as primary cell isolation, immunohistochemistry, Western blotting, cloning, mutagenesis, and transfection. Updates mouse breeding and research database; documents all procedures, tracks research supplies and sample inventory. Performs routine equipment maintenance and lab safety. Prepares standard operating procedures, writes proper analytical methods and technical information, assists with oversight, and trains students if needed. Performs other duties as assigned. Qualifications MINIMUM REQUIREMENTS: EDUCATION: Bachelor's Degree in Biology, Physiology, or in one of the sciences (TRANSCRIPT REQUIRED) EXPERIENCE: OR Combination of college course work and work experience to equal 8 years. (Previous animal handling and maintenance, genetic techniques including RNA/DNA isolation and PCR experience preferred). (Prior experience working with mice/tissue/cell isolation and culture preferred). KNOWLEDGE, SKILLS, AND ABILITIES: Strong ability to work independently with laboratory animals. Ability to perform cell cultures with strong practical experience in aseptic technique. Skills in data entry. Strict attention to detail and good organizational skills. Excellent written and oral communication skills, positive problem-solving skills. Detail oriented in animal handling and genotyping. Highly organized, computer knowledge and usage are required. Ability to stay abreast with new advances in techniques, instrumentation and technology. An official transcript or documentation to verify education must be submitted in a sealed envelope to the Human Resources Department prior to first day of employment if selected for a position.

Keywords: University of Tennessee, Memphis , Research Assistant Pediatrics, Other , Memphis, Tennessee

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