Cryo-Electron Microscopy Senior Scientist in Blue Sky Protein Kinases
Company: St. Jude Children's Research Hospital
Location: Memphis
Posted on: May 1, 2025
Job Description:
We are looking for an experienced cryo - electron microscopy
(CryoEM) senior scientist to participate in our exciting studies of
the human protein kinome. As part of the largest BlueSky
initiative, "Seeing the Invisible in Protein Kinases" at St. Jude
Children's Research Hospital, we are expanding our structural
studies of the human kinome to identify novel kinase-binding
ligands. We are looking for experienced CryoEM scientist to aid in
these efforts.The World's Brightest Minds Always Innovate - -At St.
Jude Children's Research Hospital, we know what can be achieved
when the brightest scientific minds face the fewest barriers.
That's why we provide world-class facilities. State-of-the-art
technologies. Extraordinary support. And a collaborative,
bench-to-bedside environment where you can see, firsthand, how your
science translates into survival for the children we serve. Quite
simply, at St. Jude, we encourage you to dream big and stop at
nothing when it comes to finding cures and saving children. -The
-at St. Jude is at the forefront of scientific discovery,
supporting its scientists and investigators in their research
pursuits. The Center offers an entire Single Particle Analysis
(SPA) solution and has rapidly progressed into the domain of
Cryo-Electron Tomography (CryoET). Recently, the Center acquired
cutting-edge instrumentation, including a Titan Krios G4 electron
microscope (TEM) featuring a 'cold-FEG' electron source, a
SelectrisX energy filter, and a Falcon 4i camera.Moreover, the
Center is supported by St. Jude's high-performance computing
facility with dedicated GPU and CPU nodes and a larger shared
filesystem. The research activity will be supported by Blue Sky
initiative. This role will involve working closely as part of a
multidisciplinary team including biochemists, NMR spectroscopists,
structural biologists, computational chemists, and other
biophysical specialists.Ideal Candidate:
- Critically evaluate the feasibility of Protein Kinase mediated
complexes for CryoEM studies.
- Experience in CryoEM workflow, structure determination of human
protein cell signaling complexes. Strong command of CryoEM/ET data
processing.
- Previous experience in working with a multidisciplinary team
supporting and managing multiple projects simultaneously.
- At least few years of experience in the vitrification of
biological samples using semi-automated devices such as Vitrobot
III or IV, Leica GP2 plunger.
- At least three years of experience independently operating an
Arctica, a Talas, or a Titan Krios electron microscope.
- Proficiency in SPA or tilt series data collection using
programs such as EPU, TOMO5, and SerialEM.
- Hands-on experience with image processing and using one or two
programs, such as RELION, cryoSPARC, EMAN2, and model analysis
software, such as COOT and Chimera.Key Responsibilities
- Extensive experience in applying CryoEM techniques to determine
the structure of protein complexes.
- Support the CryoEM projects of the team, by working closely
with other team members.
- Coordinate with the leaders of the project to design CryoEM
studies with specific goals objectives and experimental plan.
- Prepare grids for samples provided by researchers across the
team.
- Implement workflows that will help the researchers with their
CryoEM studies.
- Provide training to the team members on instrument operation
and sample preparation techniques.
- Independently troubleshoot, solve difficult problems, and
propose solutions related to research project design and
execution
- Work effectively in a collaborative team environment where
results are achieved through influence, cooperation, and the
incorporation of multiple points of view
- Perform additional duties as assigned, such as performing
FIB-milling. Training can be provided.
- Stay well-aware about the progress in the field of Human
kinases and should present new findings in journal clubs and
department seminars.
- Independently motivated, detail oriented and good
problem-solving ability
- Excellent organizational skills, with an ability to embrace
change and multi-task in an extremely fast-paced environment
- Excellent communications skillsMinimum Education and/or
Training:
- Bachelor's degree in relevant scientific area is required.
- PhD preferred.Minimum Experience:
- Ten (10) years relevant work experience is required with a
Bachelor's degree.
- Eight (8) years of relevant work experience is required with a
Master's degree.
- Five (5) years of relevant work experience is required with a
PhD.Have More in MemphisGet the best of big-city amenities mixed
with all the charm of a small-town feel when you live in Memphis.
From our world-renowned music scene to our eclectic mix of food and
people. Memphis is a place our employees are proud to call home.
Our region enjoys a cost of living more than 20% lower than the
national average and our state places no personal income tax on
wages. Plus, Memphians spend five hours less per year in their
daily commute compared to the national average. Learn how you can
"." -CompensationIn recognition of certain U.S. state and municipal
pay transparency laws, St. Jude is including a reasonable estimate
of the compensation range for this role. This is an estimate
offered in good faith and a specific salary offer takes into
account factors that are considered in making compensation
decisions including but not limited to skill sets, experience and
training, licensure and certifications, and other business and
organizational needs. It is not typical for an individual to be
hired at or near the top of the salary range and compensation
decisions are dependent on the facts and circumstances of each
case. A reasonable estimate of the current salary range is $104,000
- $186,160 per year for the role of Cryo-Electron Microscopy Senior
Scientist in Blue Sky Protein Kinases.Explore our exceptional !No
Search FirmsSt. Jude Children's Research Hospital does not accept
unsolicited assistance from search firms for employment
opportunities. Please do not call or email. All resumes submitted
by search firms to any employee or other representative at St. Jude
via email, the internet or in any form and/or method without a
valid written search agreement in place and approved by HR will
result in no fee being paid in the event the candidate is hired by
St. Jude.
Keywords: St. Jude Children's Research Hospital, Memphis , Cryo-Electron Microscopy Senior Scientist in Blue Sky Protein Kinases, Other , Memphis, Tennessee
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